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Extra resources for Advances in lipid research. Volume 7
M. 25% sodium tetraborate for 18 hours at 37°C. (e) After thorough washing, mount in glycerine jelly. Lecithin and sphingomyelin are stained blue-black. The reaction of protein-bound phospholipids cannot be prevented by preliminary extraction with pyridine (as recommended by Baker); under these circumstances extraction with chloroform-methanol-hydrochloric acid (66:33:1, v/v/v) may be successful. Baker's (1946, 1947) original acid hematein method was designed for use on blocks of fixed tissue which were subsequently sectioned.
The reaction of protein-bound phospholipids cannot be prevented by preliminary extraction with pyridine (as recommended by Baker); under these circumstances extraction with chloroform-methanol-hydrochloric acid (66:33:1, v/v/v) may be successful. Baker's (1946, 1947) original acid hematein method was designed for use on blocks of fixed tissue which were subsequently sectioned. The method outlined above is a modification to use on frozen sections of fixed tissue; results with it are usually as good as with the original method, except in myelin (Adams, 1965a).
Reproduced by courtesy of the editor of/. Histochem. ) # FIG. 9. Triglycérides in storage cells of bone marrow in familial hyperlipidaemic xanthomatosis. Lipase-lead sulfide, X 960. (Sternal biopsy specimen by courtesy of Dr. ) Lipid Histochemistry 21 tors and activators. In vitro densitometric studies on lipid spots impregnated on paper revealed that waxes as well as triglycérides react with the method. 5. Phosphoglycerides a. Acid Hematein Method. Chromâtes were introduced into histology as fixatives in the middle of the last century, possibly because they were extensively used at this time for hardening leather in the tanning process.